Review



protoscript ii first strand cdna synthesis kit  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    New England Biolabs protoscript ii first strand cdna synthesis kit
    Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pmc13612924-68-4-11
    Average 99 stars, based on 1843 article reviews
    protoscript ii first strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    cDNA Synthesis:

    Article Title: Gonadotropin-Inhibitory Hormone (GnIH) Induces Glycolipid Metabolic Dysfunction in Porcine Ovarian Granulosa Cells via Wnt and AMPK Signaling Pathways.
    Article Snippet: Animal reproduction is closely linked to energy metabolism, and ovarian glycolipid metabolism disorders can lead to follicular abnormalities, reduced fertility, and infertility.. Granulosa cells (GCs), as key energy suppliers in the ovary, directly influence oocyte development, hormone secretion, and reproductive function.. However, the interaction between reproductive regulatory factors and ovarian energy metabolism is unclear.

    Article Title: Discovery and characterization of Vibrio parahaemolyticus AHPND-responsive piRNAs in modulating shrimp immune response.
    Article Snippet: 31 Piwi-interacting RNAs (piRNAs) represent the largest class of small non-coding RNAs, 32 typically ranging from 24-31 nucleotides in length.. When complexed with PIWI protein, 33 piRNAs suppress expression of transposons and protein-coding genes, acting at both the 34 transcriptional or post-transcriptional levels.. While a recent study suggest that piRNAs play a 35 regulatory role in shrimp during viral infections, their involvement in antibacterial responses 36 remains unexplored.

    Article Title: CHD7 binds distinct regions in the Sox11 locus to regulate neuronal differentiation
    Article Snippet: .. 5’ GCACTCGAGTCTGTGAACTAGG 3’ ( Sox11 sense), 5’ GCGTTGTGTGCATAGCAGTC 3’ ( Sox11 antisense), 5’ CCAGGACAATGGCACTGAAT 3’ ( Hmbs sense), 5’ AAAGTTCCCCAACCTGGAAT 3’ ( Hmbs antisense) primers were used in individual reactions for first-strand cDNA synthesis with 1 μg of total RNA and ProtoScript II Reverse Transcriptase (New England Biolabs #M0368). .. Following cDNA synthesis, PCR was performed using the same primer pairs for the respective genes with Taq 5x Master Mix (New England Biolabs #M0285L).

    Article Title: Andrographolide Suppresses Influenza A Virus-Induced Pyroptosis via PI3K/AKT-Mediated Caspase-3/GSDME Inactivation.
    Article Snippet: Subsequently, RNA-seq libraries were prepared specifically from high-integrity RNA that passed quality assessments, which is a critical step for reliable transcriptomic profiling (RNA Integrity Number > 7.0) by Annoroad Gene Technology Co., Ltd. (Beijing, China). .. Briefly, after second-strand cDNA synthesis, libraries were constructed using a heat-labile uracil-DNA glycosylase enzyme protocol (New England Biolabs, Ipswich, MA, USA) and amplified by PCR; fragments of 300 ± 50 bp were selected. ..

    Article Title: Discovery of functional factorless internal ribosome entry site-like structures through virome mining
    Article Snippet: .. RT Primer extension of modified and control RNAs was performed with primer 5’ -GTGTTGTTGTTTTGTTGTTTTCGTTG-3’ using Superscript III Reverse Transcriptase (SSIII, Thermo Fisher Scientific), with Mn2 + ; followed by second strand cDNA synthesis (NEB #E6111). ..

    Article Title: CapTrap-seq: Advancing zebrafish transcriptomic research through high-fidelity full-length RNA sequencing
    Article Snippet: Samples were quantified with Qubit (Thermo Fisher Scientific). .. 4 μl of ribo-depleted RNA per reaction was used for first-strand cDNA synthesis with Template Switching RT Enzyme Mix (NEB, Cat. No. M0466), Template Switching Oligo (75 μM; 5’AAGCAGTGGTATCAACGCAGAGTACATrGrG+G3’), and anchored oligo-dT (10 μM; 5’AAGCAGTGGTATCAACGCAGAGTACT30VN3’). ..

    Article Title: Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.
    Article Snippet: .. Reverse transcription was performed by first-strand cDNA synthesis from RNA (ProtoScript II First Strand cDNA Synthesis kit, NEB), which was then used as a template for RT-qPCR with the SYBR PowerUp Master Mix (Applied Biosystems) on a Roche LightCycler 480 Real-time PCR system. ..

    Reverse Transcription:

    Article Title: Gonadotropin-Inhibitory Hormone (GnIH) Induces Glycolipid Metabolic Dysfunction in Porcine Ovarian Granulosa Cells via Wnt and AMPK Signaling Pathways.
    Article Snippet: Animal reproduction is closely linked to energy metabolism, and ovarian glycolipid metabolism disorders can lead to follicular abnormalities, reduced fertility, and infertility.. Granulosa cells (GCs), as key energy suppliers in the ovary, directly influence oocyte development, hormone secretion, and reproductive function.. However, the interaction between reproductive regulatory factors and ovarian energy metabolism is unclear.

    Article Title: CHD7 binds distinct regions in the Sox11 locus to regulate neuronal differentiation
    Article Snippet: .. 5’ GCACTCGAGTCTGTGAACTAGG 3’ ( Sox11 sense), 5’ GCGTTGTGTGCATAGCAGTC 3’ ( Sox11 antisense), 5’ CCAGGACAATGGCACTGAAT 3’ ( Hmbs sense), 5’ AAAGTTCCCCAACCTGGAAT 3’ ( Hmbs antisense) primers were used in individual reactions for first-strand cDNA synthesis with 1 μg of total RNA and ProtoScript II Reverse Transcriptase (New England Biolabs #M0368). .. Following cDNA synthesis, PCR was performed using the same primer pairs for the respective genes with Taq 5x Master Mix (New England Biolabs #M0285L).

    Article Title: Discovery of functional factorless internal ribosome entry site-like structures through virome mining
    Article Snippet: .. RT Primer extension of modified and control RNAs was performed with primer 5’ -GTGTTGTTGTTTTGTTGTTTTCGTTG-3’ using Superscript III Reverse Transcriptase (SSIII, Thermo Fisher Scientific), with Mn2 + ; followed by second strand cDNA synthesis (NEB #E6111). ..

    Article Title: Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.
    Article Snippet: .. Reverse transcription was performed by first-strand cDNA synthesis from RNA (ProtoScript II First Strand cDNA Synthesis kit, NEB), which was then used as a template for RT-qPCR with the SYBR PowerUp Master Mix (Applied Biosystems) on a Roche LightCycler 480 Real-time PCR system. ..

    other:

    Article Title: Discovery of functional factorless internal ribosome entry site-like structures through virome mining.
    Article Snippet: PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013255 June 26, 2025 22 / 30 In vitro translation assays In vitro transcribed RNAs were incubated in rabbit reticulocyte lysates (RRL) or Sf21 insect lysates (Promega) for 1 hour at 37C or 30C in the presence of [35S]-methionine as described previously [78].

    RNA Extraction:

    Article Title: Discovery and characterization of Vibrio parahaemolyticus AHPND-responsive piRNAs in modulating shrimp immune response.
    Article Snippet: 31 Piwi-interacting RNAs (piRNAs) represent the largest class of small non-coding RNAs, 32 typically ranging from 24-31 nucleotides in length.. When complexed with PIWI protein, 33 piRNAs suppress expression of transposons and protein-coding genes, acting at both the 34 transcriptional or post-transcriptional levels.. While a recent study suggest that piRNAs play a 35 regulatory role in shrimp during viral infections, their involvement in antibacterial responses 36 remains unexplored.

    Quantitative RT-PCR:

    Article Title: Discovery and characterization of Vibrio parahaemolyticus AHPND-responsive piRNAs in modulating shrimp immune response.
    Article Snippet: 31 Piwi-interacting RNAs (piRNAs) represent the largest class of small non-coding RNAs, 32 typically ranging from 24-31 nucleotides in length.. When complexed with PIWI protein, 33 piRNAs suppress expression of transposons and protein-coding genes, acting at both the 34 transcriptional or post-transcriptional levels.. While a recent study suggest that piRNAs play a 35 regulatory role in shrimp during viral infections, their involvement in antibacterial responses 36 remains unexplored.

    Article Title: Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.
    Article Snippet: .. Reverse transcription was performed by first-strand cDNA synthesis from RNA (ProtoScript II First Strand cDNA Synthesis kit, NEB), which was then used as a template for RT-qPCR with the SYBR PowerUp Master Mix (Applied Biosystems) on a Roche LightCycler 480 Real-time PCR system. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Discovery and characterization of Vibrio parahaemolyticus AHPND-responsive piRNAs in modulating shrimp immune response.
    Article Snippet: 31 Piwi-interacting RNAs (piRNAs) represent the largest class of small non-coding RNAs, 32 typically ranging from 24-31 nucleotides in length.. When complexed with PIWI protein, 33 piRNAs suppress expression of transposons and protein-coding genes, acting at both the 34 transcriptional or post-transcriptional levels.. While a recent study suggest that piRNAs play a 35 regulatory role in shrimp during viral infections, their involvement in antibacterial responses 36 remains unexplored.

    Article Title: Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.
    Article Snippet: .. Reverse transcription was performed by first-strand cDNA synthesis from RNA (ProtoScript II First Strand cDNA Synthesis kit, NEB), which was then used as a template for RT-qPCR with the SYBR PowerUp Master Mix (Applied Biosystems) on a Roche LightCycler 480 Real-time PCR system. ..

    Construct:

    Article Title: Andrographolide Suppresses Influenza A Virus-Induced Pyroptosis via PI3K/AKT-Mediated Caspase-3/GSDME Inactivation.
    Article Snippet: Subsequently, RNA-seq libraries were prepared specifically from high-integrity RNA that passed quality assessments, which is a critical step for reliable transcriptomic profiling (RNA Integrity Number > 7.0) by Annoroad Gene Technology Co., Ltd. (Beijing, China). .. Briefly, after second-strand cDNA synthesis, libraries were constructed using a heat-labile uracil-DNA glycosylase enzyme protocol (New England Biolabs, Ipswich, MA, USA) and amplified by PCR; fragments of 300 ± 50 bp were selected. ..

    Amplification:

    Article Title: Andrographolide Suppresses Influenza A Virus-Induced Pyroptosis via PI3K/AKT-Mediated Caspase-3/GSDME Inactivation.
    Article Snippet: Subsequently, RNA-seq libraries were prepared specifically from high-integrity RNA that passed quality assessments, which is a critical step for reliable transcriptomic profiling (RNA Integrity Number > 7.0) by Annoroad Gene Technology Co., Ltd. (Beijing, China). .. Briefly, after second-strand cDNA synthesis, libraries were constructed using a heat-labile uracil-DNA glycosylase enzyme protocol (New England Biolabs, Ipswich, MA, USA) and amplified by PCR; fragments of 300 ± 50 bp were selected. ..

    Polymerase Chain Reaction:

    Article Title: Andrographolide Suppresses Influenza A Virus-Induced Pyroptosis via PI3K/AKT-Mediated Caspase-3/GSDME Inactivation.
    Article Snippet: Subsequently, RNA-seq libraries were prepared specifically from high-integrity RNA that passed quality assessments, which is a critical step for reliable transcriptomic profiling (RNA Integrity Number > 7.0) by Annoroad Gene Technology Co., Ltd. (Beijing, China). .. Briefly, after second-strand cDNA synthesis, libraries were constructed using a heat-labile uracil-DNA glycosylase enzyme protocol (New England Biolabs, Ipswich, MA, USA) and amplified by PCR; fragments of 300 ± 50 bp were selected. ..

    Modification:

    Article Title: Discovery of functional factorless internal ribosome entry site-like structures through virome mining
    Article Snippet: .. RT Primer extension of modified and control RNAs was performed with primer 5’ -GTGTTGTTGTTTTGTTGTTTTCGTTG-3’ using Superscript III Reverse Transcriptase (SSIII, Thermo Fisher Scientific), with Mn2 + ; followed by second strand cDNA synthesis (NEB #E6111). ..

    Control:

    Article Title: Discovery of functional factorless internal ribosome entry site-like structures through virome mining
    Article Snippet: .. RT Primer extension of modified and control RNAs was performed with primer 5’ -GTGTTGTTGTTTTGTTGTTTTCGTTG-3’ using Superscript III Reverse Transcriptase (SSIII, Thermo Fisher Scientific), with Mn2 + ; followed by second strand cDNA synthesis (NEB #E6111). ..



    Similar Products

    96
    TaKaRa primescript 1 st strand cdna synthesis kit
    Primescript 1 St Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/PrimeScript+Double+Strand+cDNA+Synthesis+Kit/pmc13634210-136-5-12
    Average 96 stars, based on 1 article reviews
    primescript 1 st strand cdna synthesis kit - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    99
    New England Biolabs protoscript ii first strand cdna synthesis kit
    Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pmc13612924-68-4-11
    Average 99 stars, based on 1 article reviews
    protoscript ii first strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    TaKaRa primescripttm ii 1st strand cdna synthesis kit
    Primescripttm Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc13123502-115-29-36
    Average 99 stars, based on 1 article reviews
    primescripttm ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    86
    Yeasen Biotechnology hifair ii 1st strand cdna synthesis supermix for qpcr
    Hifair Ii 1st Strand Cdna Synthesis Supermix For Qpcr, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/1st+cdna+for+hifair+iii+qpcr+strand+supermix+synthesis/pmc13141777-125-6-15
    Average 86 stars, based on 1 article reviews
    hifair ii 1st strand cdna synthesis supermix for qpcr - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    99
    TaKaRa primescripttm rt mix
    Primescripttm Rt Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/PrimeScript+IV+1st+strand+cDNA+Synthesis+Mix/pm42816477-371-4-7
    Average 99 stars, based on 1 article reviews
    primescripttm rt mix - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs complementary dna cdna synthesis
    Complementary Dna Cdna Synthesis, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pm42793113-91-0-19
    Average 99 stars, based on 1 article reviews
    complementary dna cdna synthesis - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    96
    TaKaRa primescripttm ii firststrand cdna synthesis kit
    Primescripttm Ii Firststrand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/PrimeScript+Double+Strand+cDNA+Synthesis+Kit/pm42791738-70-15-21
    Average 96 stars, based on 1 article reviews
    primescripttm ii firststrand cdna synthesis kit - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology first strand cdna synthesis kit
    First Strand Cdna Synthesis Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/ABscript+II+cDNA+First+Strand+Synthesis+kit/pm42789758-75-12-16
    Average 98 stars, based on 1 article reviews
    first strand cdna synthesis kit - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology reverse transcription kit
    Reverse Transcription Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/ABscript+II+cDNA+First+Strand+Synthesis+kit/pm42791352-263-19-22
    Average 98 stars, based on 1 article reviews
    reverse transcription kit - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    96
    Vazyme Biotech Co hiscript iv 1st strand cdna synthesis kit
    Hiscript Iv 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis/HiScript+IV+1st+Strand+cDNA+Synthesis+Kit+%2BgDNA+wiper/pm42815083-232-17-24
    Average 96 stars, based on 1 article reviews
    hiscript iv 1st strand cdna synthesis kit - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results